EFFECTS OF GENISTEIN ON APOPTOSIS IN HUMAN OSTEOBLAST CELLS / 营养学报
Acta Nutrimenta Sinica
;
(6)1956.
Article
in Chinese
| WPRIM
| ID: wpr-555334
ABSTRACT
Objective:
To investigate the effects of genistein (Gen) on apoptosis in human osteoblast.Methods:
Human periosteum was maintained in DMEM medium containing 10% newborn calf serum through four passages to isolate and proliferate osteoblast. Then, cells were maintained in phenol red-free DMEM supplemented with 5% charcoal-stripped FCS for 4 d before addition of treatments. Flow cytometer and DNA fragment were used to explore apoptosis in osteoblast, and immunohistochemistry was used to study protein expression of Bax, Bcl-2 and caspase-3.Results:
Estrogen depletion could induce apoptosis in human osteoblast. With the similarity of 10-8 mol/L E2, at the conentrations of 10-6 mol/L and 10-5 mol/L, Gen could inhibit apoptosis induced by estrogen depletion. Compared with vehicle control, Gen significantly decreased protein expression of Bax and caspase-3 and increased protein expression of Bcl-2.Conclusion:
Genistein could inhibit apoptosis induced by estrogen depletion in human osteoblast.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Acta Nutrimenta Sinica
Year:
1956
Type:
Article
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