Protective effect of baicalin-mediated on mice spinal ganglion cells against gentamicin-induced ototoxicity / 中国临床药理学与治疗学

ABSTRACT

AIM: To study the protective effect of baicalin (BA) against the ototoxicity of gentamicin (GM) of mice spinal ganglion (SG) cells. METHODS: MTT assay was employed to determine the growth inhibition of GM on the SG cells. The activities of superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-PX), and the content of malondialdehyde (MDA) were measured by UV chromatography in SG cells from mice of different groups. RESULTS:Baicalin reversibly limited the GM-induced growth inhibition of SG cells in a dose-dependent manner. BA increased the activity of T-SOD and GM-induced decreased activity of T-SOD and GSH-PX in SG cells. The compound could also reduce MDA level and limite the GM-induced high content of MDA in SG cells. CONCLUSION: Baicalin mediated neuroprotective effect on SG cells is against gentamicin-induced ototoxicity through anti-oxidant.