Potential effect and mechanism of IFN? on tumor angiogenesis / 中国临床药理学与治疗学
Chinese Journal of Clinical Pharmacology and Therapeutics
;
(12)2004.
Article
in Chinese
| WPRIM
| ID: wpr-555352
ABSTRACT
AIM:
To study the effect and potential mechanism of IFN? on tumor angiogenesis.METHODS:
Human umbilical cord vascular endothelial cell (HUVEC) was cultured in vitro. The effect of IFN? (10, 100, and 1000 unit?ml -1) on proliferation of HUVEC was detected by MTT assay in vitro. HUVEC pretreated with IFN? (10, 100, and 1000 unit?ml -1) was stained with PI and detected by flow cytometry (FCM). The mRNA expression of apoptosis relative gene Bcl-2 and Bax of HUVEC treated with IFN? (100, and 1 000 unit?ml -1) were detected with semi quantitative reverse transcription- polymerase chain reaction (RT-PCR). IFN? (30, 300, and 3 000 unit?ml -1) on VEGF secretion and expression in human lung carcinoma cell PG in hypoxia were detected by ELISA assay.CONCLUSION:
The key attributes of IFN? on tumor angiogenesis possible directly inhibit vascular endothelial cells or indirectly inhibit growth factors secretion and expression in tumor cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Clinical Pharmacology and Therapeutics
Year:
2004
Type:
Article
Similar
MEDLINE
...
LILACS
LIS