Effect to the expression of VEGF in K562 cells by down-regulation of WT1 / 肿瘤研究与临床

ABSTRACT

Objective To investigate the effect to the expression of VEGF in human leukemic cell line by WT1gene silencing.MethodsK562 cells were transfected with WT1shRNA Plasmid.Transfection efficiency was detected by flow cytometIy,and the succeed transfered cells Were sorted.WT1 and VEGF mRNA variation were measured by fluorescence RT-PCR.VEGF concentrations in the cell growth medium were detected by ELISA.Results 48 hours after transfection,the transfection efficiency was 65％±4.5％.WT1shRNA positive cells were sorted by flow cytometry.Both the mRNA level of WT1 and VEGF and VEGF concentrations in cell growth medium were significantly decreased than the control group.ConclusionWT1 gene silence can down-regulate the expression of VEGF in K562 cells which suggest that WT1 gene may take part in leukemia angiogenesis through up-regulation of VEGF.