Effects of compound stanozolol in prednisone-induced osteoporosis rats / 中国药理学通报

ABSTRACT

Aim To study the characteristics of osteoporosis induced by long-term administration of prednisone, and investigate the preventive effects of compound stanozolol (CS) on the adverse reactions in male rats. Methods Twenty-four four-month-old male Sprague-Dawley rats were randomly divided into 3 groups with 8 rats per group. Group 1 was control (NS group), other groups were oral gavages prednisone (2.7 mg?kg -1?d -1) first, and then plus vehicle (GC group) or plus CS (combination of Stanozolol 0.5 mg?kg -1?d -1 + Calciofon 0.5 g?kg -1?d -1 + Vitamin D 3 250 U?kg -1?d -1), once a day for 12 weeks. At the experimental endpoint, animals were drawn blood from right ventricle under anesthesia. The tibia, ulna and thighbone were collected to test for parameters related to bone.The undecalcified longitudinal proximal tibia metaphyseal sections were cut and stained with Masson-Goldner's Trichrome (5-?m thickness) for osteoclasts analysis, unstained sections (8-?m thickness) for the cancellous bone histomorphometric and fluorochrome labeling analysis. The measured parameters were used to calculate the percent trabecular area (Tb.Ar%), trabecular thickness (Tb.Th), trabecular number (Tb.N), trabecular separation (Tb.Sp), percent labeled perimeter (L.Pm%), Mineral apposition rate (MRA), bone formation rate (BFR/TV, BFR/BV, BFR/BS), unit area osteoclast number (Oc.N/BV) and Percent osteoclast number surface perimeter (OcP/BS). The desiccant left ulnar was dissolved in hydrochloric acid to test the content of Ca 2+ and hydroxyproline. Length and width of the thigh bone were tested to observe the instance of bone growing. Results Compared with NS group, bone weight, contents of bone hydroxyproline and Ca 2+decreased remarkably in GC group and the bone histomorphometric parameters also showed bone lose significantly.The effects of CS in prednisone rats showed that CS prevented bone loss effectively.Conclusions Bone loss occured in four-month-old male Sprague-Dawley rats after prednisone-treated for 12 weeks. CS could prevent bone loss effectively by inhibiting bone resorption and advancing bone formation.