Effects of high glucose and losartan on the expression of Smad in human peritoneal mesothelial cells / 中华肾脏病杂志

ABSTRACT

Objective To investigate if the mechanism of high glucose and losartan in mediating the expression of Smad in human peritoneal mesothelial cells (HPMC) and the possible management of HPMC. Methods Peritoneum was obtained from patients undergoing elective abdominal surgery. HPMCs were incubated in medium containing different concentrations of dextrose, mannitol (1. 5%, 2. 5% , 4. 25% ) and combination with dextrose and losartan. TGF-?1 in supernatant was detected by ELISA and HPMCs were collected to examine Smad family expression with RT-PCR and Western Blot. Results (1) High glucose up-regulated the expression of Smad 2 at both gene and protein levels, especially in 2. 5% and 4. 25% dextrose groups (P