The effect of hesperdin and synephrine on the isolated hastric smooth muscle cells / 中国药理学通报

ABSTRACT

Aim To study the effect of hesperdin and synephrine on the isolated gastric smooth muscle cells.Method Gain isolated gastric smooth muscle cells by digesting gastric smooth muscle with collagenase(typeⅡ), measure long axis length of 25～50 cells randomly under microscope. Then, compare the contraction change of cells between before and after given drugs, the contraction change of cells is expressed with percent, negative expresses contraction, positive expresses relaxation.Results Hesperdine had no effect on the isolated gastric smooth muscle cells. The contractile response to synephrine was prompt, rising to a peak within 60 s and dose-dependent at the range of 0.001～1 g?L -1. Synephrine inhibited the excited cells caused by acetylcholine(P