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Isolation, purification and identification of nucleosomes from chicken erythrocytes / 第三军医大学学报
Journal of Third Military Medical University ; (24)2003.
Article in Chinese | WPRIM | ID: wpr-555892
ABSTRACT
Objective To establish a simple and practical method for isolating and purifying nucleosomes. Methods Nuclei were isolated from chicken erythrocytes, and then digested with staphylococcal nuclease. After centrifugation, the supernatant of digestion was separated and centrifugated on sucrose gradients. Results Nucleosomes with good stability were isolated properly by gradient centrifugation. Conclusion This method for the isolation and purification of nucleosomes is simple and effective, which might contribute to the further researches of the roles of nucleosomes in the pathogenesis of systemic lupus erythematosus.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study Language: Chinese Journal: Journal of Third Military Medical University Year: 2003 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study Language: Chinese Journal: Journal of Third Military Medical University Year: 2003 Type: Article