Effects of SWH solution on hepatic energy metabolism in rats / 第三军医大学学报

ABSTRACT

Objective To investigate the effects of SWH solution on hepatic energy metabolism in SD rats compared with UW solution. Methods Levels of ATP, ADP, and AMP in hepatic tissues were examined by high performance liquid chromatography (HPLC) while ALT, AST, and LDH were examined and the 7-day survival rate of animals was observed with orthotopic liver transplantation model in rats. Results Levels of ATP, TAN, and EC in 18-h group were significant lower than those in 8-h group. No significant difference was found between 12-h group and 8-h group. AST, ALT, and LDH were significantly increased after preservation for 18-h than preservation for 8-h. No significant difference was found between 12-h group and 8-h group. The 7-d survival rate of animals in SWH group was higher than that in UW group. The change tendency of ATP, TAN, and EC was the same as the tendency of 7-d survival rate of animals. Conclusion These results suggest that levels of ATP, ADP, and AMP in hepatic tissues are closely associated with the survival rate, and SWH solution is effective in protecting hepatic energy metabolism.