Fermentation technology in heat-labile enterotoxin B subunit of genetic recombinant E. coli / 第三军医大学学报
Journal of Third Military Medical University
;
(24)1984.
Article
in Chinese
| WPRIM
| ID: wpr-556009
ABSTRACT
Objective To investigate the fermentation conditions for the heat-labile enterotoxin B subunit (LTB) of genetic recombinant E.coli. Methods The effects of different kinds of media, the range of pH, the induction time, the glucose concentration, and the fed-batch on LTB level in 10-liter fermenter under the condition of cascade controlling dissolved oxygen content were analyzed. Results Under the established conditions, the cell output per liter was 40.5g, and the expression level was 30.6%. Conclusion The results indicate that the stable fermentation technology with high efficiency has been established.
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Third Military Medical University
Year:
1984
Type:
Article
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