Effects of aldosterone on nos-no system activity of cardiac fibroblasts in neonatal rat / 解放军医学杂志

ABSTRACT

Objective To investigate the changes in nitric oxide synthase (NOS) activity in cardiac fibroblasts (CFs) of neonatal rat induced by aldosterone. Method CFs were isolated by trypsin digestion method. Proliferation of CF was assessed by MTT colorimetric assay. Nitric acid reductase method and spectrophotometry were used to determine the NO contents and NOS activity, respectively, at different time after CFs being treated with aldosterone. Result Aldosterone (1?10 -11 -1?10 -7 mol/L) could increase CFs proliferation in a dose-dependent manner. NO contents, NOS activity and iNOS mRNA were decreased in a dose-dependent manner after being treated with aldosterone in different concentrations (P