Protective effect of Ginkgo biloba L. extract on PC12 cell damage induced by rotenone and MPP~+ in vitro / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12)1982.
Article
in Chinese
| WPRIM
| ID: wpr-556112
ABSTRACT
Objective:
To study the protective effect of Ginkgo biloba L.extract(EGB) on PC12 cell damage induced by rotenone and 1-methyl-4-phenylpyridium(MPP +).Methods:
PC12 cells damage model was induced with different concentrations of rotenone and MPP + and were treated with EGB . Then cell morphology was observed; survival rate and ultracellular dopamine (DA) level were determined by MTT assay and ELISA assay, respectively.Results:
MPP + -induced damage was attenuated by pre- or co-treatment with EGB at 0.35,0.70 and 1.40 mg/ml, while rotenone-induced damage was attenuated with EGB only at 1.40 mg/ml.The DA concentrations of extracellular at 2 mmol/L MPP + (6.875?0.201) ng/ml and 10 ?mol/L rotenone (5.321?0.167) ng/ml increased to ( 7.595?0.139 ) ng/ml (P
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
1982
Type:
Article
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