The effects of aminophylline on mRNA expression of interleukin-5, interleukin -3 and granulocyte-macrophage colony-stimulating factor receptors in eosinop hils of asthmatic guinea pigs / 中国药理学通报

ABSTRACT

Aim To investigate the effect of Aminophyllin e(AM) on expression of Interleukin-5 receptor ?(IL-5R?), IL-3R?, granulocy te- macrophage-colony-stimulating factor receptor ?(GM-CSFR?) and common ? receptor(?cR) in eosinophils(Eos) of BALF in asthmatic guinea pigs, and mech anism of AM promoting Eos apoptosis. Methods 18 guinea pigs wer e divided into three groups randomly, normal control group, asthma group and AM -treated group. Asthma model of guinea pigs was sensitized by ovalbumin. Hypode nse Eos(HEos) and normodense Eos(NEos) were purified from BALF by gradients of p ercoll. Apoptosis was detected by TUNEL. mRNA expression of IL-5R?, IL-3R?, GM-CSFR? and ?cR in Eos were measured by RT-PCR and hybridization.Re sults Apoptosis of HEos and NEos in asthma group(4?2,3?2) were low er than that in normal group(8?2,7?2)(P