The significance of united mutations in precore and BCP of HBV in patients with HBeAg-negative hepatitis B / 中华传染病杂志

ABSTRACT

Objective To illustrate the relationship between the serum viral load, clinical types and the G1896A in precore, A1762T/G1764A in basal core promoter (BCP) or united mutation of them of HBV from patients with HBeAg-negative hepatitis. Methods Sera from 240 HBeAg-negative, 60 HBeAg-positive patients, and 40 negative controls were collected. Mutations of G1896A and A1762T/G1764A were detected using CD-PCR. Viral load in sera was demonstrated using real-time quantification PCR. Results The mutations of G1896A were detected in 57.6% and 6.7% in HBeAg-negative and HBeAg-positive patients respectively. The mutations of A1762T/G1764A were detected in 37.9% and 31.7% in the same patients respectively. The united mutations were detected in 13.5% of HBeAg-negative patients. G1896A was associated with low viral load, and A1762T/G1764A did not have any special relation viral load in sera from HBeAg-negative patients. The united mutations often occurred in patients with severe chronic hepatitis B, and been not related to the viral load. Conclusions The replication capability of G1896A variant decreased without HBeAg production as usual. For A1762T/G1764A variant, the situation of its replication activity was complicated. Its HBeAg production was less influenced than that of G1896A variant. The united variant might has higher pathogenicity and replication activity, and to which much attention should be paid in the future.