Experimental study on the change in intracellular glycogen in ovarian cancer after all-trans retinoic acid treatment / 解放军医学杂志

ABSTRACT

Objective To investigate the effect of all-trans retinoic acid on the change in intracellular glycogen in ovarian epithelioma cell line in vitro and ovarian epithelial carcinoma in nude mice in vivo. Methods COC2 cells were treated with all-trans retinoic acid in 1, 5, 10 and 30?mol/L drug concentrations for different length of time, and then intracellular glycogen and LDH were determined by biochemistry assay. Morphologic changes were observed with light and electron microscopy. CAOV3 tumor-bearing nude mice were treated with intragastric injection of the same drug in a dose of 2mg/(kg?d) for four weeks. The tumor samples were harvested thereafter for pathological study with histochemical and immunohistochemical staining, and also with electron microscopy. Results Intracellular glycogen was significantly increased, while LDH level was lowered after the cell line was treated with 5~10?mol/L of all-trans retinoic acid, and apoptosis of cancer cell occurred after using 30?mol/L of the drug. These changes were also observed in CAOV3 cells of tumor-bearing nude mice. Conclusion Our results suggest that treatment with all-trans retinoic acid resultin an increase in intracellular glycogen and decrease in LDH level both in COC2 cells in vitro and in CAOV3 tumor-bearing nude mice in vivo, and the suppression of tumor cell proliferation may be attributed to retarded intracellular metabolism.