Isoflurane increase renal artial smooth muscle contraction may through phosphorylated ERK1/2 pathway / 中国药理学通报

ABSTRACT

Aim This study was to investigate the effect of isoflurane on contracted renal arterial smooth muscle and to determine whether the MAPK pathway is involved in the intracellular signaling. Methods ①Rabbit renal arterial strips were treated with sapoin to make the sarcolemma permeability. And the calcium stored in reticulum (SR) was released using caffeine.Stead state tense was obtained when the strips were soaked in submaximal calcium concentration buffer.Then the submaximal calcium concentration buffer with various concentration isoflurane was admininstered,the change of tensions was detected.②primary renal arterial smooth muscle cells were cultured and treated with different concentration of isoflurane for different duration,and the levels of phosphorylated ERK1/2 in different groups were detected with western blot.Result Isoflurane all caused rabbit renal artery strips further contraction after they have reached equilibrium,and the tension altitude was isoflurane concentration dependent. Isoflurane upregulates ERK1/2 (p44/42) phosphorylation in cultured renal arterial smooth muscle cells in a concentration and time depend way.Conclusion Isoflurane can upregulates the tension of contracted renal arterial smooth muscle. And it may involve the activation of p44/42 MAPK pathway.