Effect of mast cell tryptase on PAR-2 and MCP-1 mRNA expression of human renal fibroblasts in culture / 解放军医学杂志

ABSTRACT

Objective To further investigate the effect of mast cell tryptase on the pathogenesis of chronic renal fibrosis. Methods Renal interstitial fibroblasts were isolated from histologically normal renal tissue obtained from kidneys removed because of malignant renal tumor by collagenase disintegration, and cultured in vitro. The cultured fibroblasts were identified by cell shape and immunocytochemistry, and divided into groups for further experiments. The expression of monocyte chemoattractant protein-1 (MCP-1) and protease-activated receptor 2(PAR-2) mRNAs in in vitro cultured fibroblasts was assessed by means of semi-quantitive reverse transcriptase polymerase chain reaction (RT- PCR). Result Renal fibroblasts were successfully cultured. Mast cell tryptase(10~500ng/ml) promoted expression of PAR-2 mRNA and MCP-1 mRNA of human renal fibroblasts in heparin-dependent manner. Proteinase inhibitor benzamidine hydrochloride hydrate (200?mol/L) could suppress the effects of tryptase on the cultured renal fibroblasts. But TGF-? antibody did not influence the effect. Conclusion Tryptase might be involved in pathogenesis and development of renal fibrosis by PAR-2 and MCP-1.