Establishment of a model of retinal microglial cells activation in vitro / 北京大学学报(医学版)
Journal of Peking University(Health Sciences)
;
(6)2004.
Article
in Chinese
| WPRIM
| ID: wpr-556344
ABSTRACT
Objective:
To develop a method of retinal microglial cell culture to study the function of the microglial cell in diabetic retinopathy.Methods:
Microglia were activated with LPS. Immunocytochemistry, con-focal microscopy, flow cytometry, MTT and ELISA were applied to observe the morphological characters, quantity, and functional changes of the microglia.Results:
The purity of the microglia was up to 96% as determined by immunostaining and flow cytometry. Some morphological changes of microglia were observed after treatment with LPS, but their quantity kept stable. Cytokine TNF-? released from microglia increased significantly.Conclusion:
The isolated microglial cells are pure by using this culture system, which would provide a valuable tool for studying mechanisms of microglial alterations in diabetic retinopathy.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
Journal of Peking University(Health Sciences)
Year:
2004
Type:
Article
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