Your browser doesn't support javascript.
loading
Proteome analysis of nuclear matrix proteins during arsenic trioxide induced apoptosis in K562 cells / 北京大学学报(医学版)
Journal of Peking University(Health Sciences) ; (6)2004.
Article in Chinese | WPRIM | ID: wpr-556353
ABSTRACT

Objective:

To investigate arsenic trioxide (As 2O 3) -target interactions at the level of nuclear matrix (NM) in chronic myelogenous leukemia cell line K562 by proteomics.

Methods:

DNA fragmentation analysis was used for As 2O 3 induced apoptosis of K562 cells. The nuclear matrix proteins were analyzed by high-resolution two-dimensional gel electrophoresis and computer-assisted image analysis.

Results:

While more than 200 protein spots were shared among the nuclear matrices, about 18 distinct spots were found characteristic of As 2O 3 treated cells. Onset of mass mange apoptosis, and the profiling of nuclear matrix proteins had been alternated and it was a more sensitive indicator than nucleosomal DNA fragmentation against As 2O 3 treatment.

Conclusion:

As 2O 3 induced apoptosis in K562 cells in a dose-time-dependent manner. As 2O 3 might be clinically useful in treatment of chronic myelogenous leukemia and the changes of nuclear matrix proteins in the treated cells can be used as a useful indicator for the treatment.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Peking University(Health Sciences) Year: 2004 Type: Article

Similar

MEDLINE

...
LILACS

LIS

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: Journal of Peking University(Health Sciences) Year: 2004 Type: Article