Construction and activity determination of modified prostatic specific promoters rPB-DRs / 第三军医大学学报

ABSTRACT

Objective To construct the prostatic specific promoter used to induce the gene therapy aiming at androgen independent prostatic carcinoma and evaluate the activity. Methods Through two steps of polymerase chain reaction (PCR), androgen response elements (AREs) in rPB were replaced by synthetic retinoic acid response elements (RAREs). Then the modified rPBs were connected with the plasmid. The activity was evaluated. Results Constructions were confirmed to be successful by electrophoresis and gene sequencing. Conclusion Modified rPB should be the prostatic specific promoter which can induce the gene therapy aiming at androgen-independent prostatic carcinoma controlled by retinoic acid. After being constructed, the modified rPBs were used to confirm our ideas about the experiment design and the capability inducing the gene expression of different modified rPBs was compared.