Protein kinase A signal pathway regulating corticotropin-releasing hormone mRNA expression in hypothalamic slice of rats in vitro / 第三军医大学学报
Journal of Third Military Medical University
;
(24)2003.
Article
in Chinese
| WPRIM
| ID: wpr-556564
ABSTRACT
Objective To investigate protein kinase A (PKA) signal regulatory mechanism of corticotropin-releasing hormone (CRH) mRNA expression, after CRH stimulated neuron of hypothalamic slices in rats in vitro. Methods After CRH stimulated corticotropin-releasing hormone type 1 receptor (CRH1R) of hypothalamic slices in rats in vitro, the changes of activity of PKA signal pathway and their relationship with CRH mRNA expression were observed by reverse transcription-polymerase chain reaction (RT-PCR), Western blotting. Results CRH may cause the remarkable increase in phosphorylated PKA (P-PKA), phosphorylated cyclic adenosine monophosphate response element-binding protein (P-CREB) and CRH mRNA content in hypothalamic slices in rats. However, CP-l54526 or H89 could have significant inhibition effects on the synthesis of P-PKA, P-CREB and CRH. Conclusion PKA signal pathway in ultrashort positive feedback control of CRH secretion in hypothalamus in the stress due to severe traumas.
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Third Military Medical University
Year:
2003
Type:
Article
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