Effect of Glycine on endotoxin induce myocardial cell injury of isolated rat heart / 中国药理学通报

ABSTRACT

Aim To observe the protective effect of glycine from myocardial cell injury of the isolated rat heart induced by endotoxin. Methods 21 male SD rats,weghting(250?30)g,were randomly divided into three groupscontrol group(n=7),endotoxin group(n=7),glycine/endotoxin group(n=7).Heart were isolated from the rats and perfused on Langendorff apparatus with Krebs-Henseleit(KH)buffer(Saturation 95%+5% CO_2)at a constant pressure(8.33 kPa)and temperture(37℃). The activities of lactate dehydrogenase(LDH),creatine kinase(CK),superoxide dismutase(SOD) and the level of malondialdehyde (MDA) of efflux from coronary artery, monophasic action potential(MAP)were determined at the certain time(0,20,50,80 min). Results Monophasic action potential were markedly improved in Gly/ET group. Glycine can attenuate the release of LDH,CK from myocardial cell. The activity of SOD and the level of MDA were close to control group. Conclusion Glycine can protect myocardial cells of the isolated rat heart from the damage induced by endotoxin.