PPAR-? ligand in suppressing radiation induced PAI-1 upregulation / 中华放射肿瘤学杂志

ABSTRACT

Objective observe the post-radiation expression of rat mesangial cell plasminogen activator inhibitor-1 (PAI-1), and the effect of PPAR-? ligand on radiation induced PAI-1 expression. Methods After 10?Gy ?-ray radiation of cultured rat kidney mesangial cell, RT-PCR was used to observe PPAR-?expression. PAI-1 expression after 10?Gy radiation ?10?mmol/L pioglitazone (one of PPAR-? ligands) was measured by Northern blot and Western blot. Gel-shift method was used to study the effect of AP-1 of PPAR-? ligand on radiation induced PAI-1 expression. Methods Rat kidney mesangial cell expressed PPAR--? mRNA. PAI-1 over-expressed at 24 hour after 10?Gy radiation in a radiation dose dependent manner, but not at other time. When the cell was treated with 10?Gy + 10?mmol/L pioglitazone, PAI-1 upregulation was blunted at message and protein level. AP-1 activation was increased from the 2nd hour after 10?Gy radiation and reached the highest level at the 4th hour. When pioglitazone was added, the radiation induced increase in AP-1 activation was suppressed at the 4th hour. Conclusions Pioglitazone, mediated by AP-1 gene, can suppress radiation induced PAI-1 upregulation.