The gene expression profiles in cardiac muscles by DNA microarray after electrical injury / 第三军医大学学报

ABSTRACT

Objective To study the gene expression profiles in cardiac muscles at the early phase after electrical injury. Methods The total RNA in both injured group and control group were isolated and hybridized with BioStar-40 cDNA microarray that contains 4 096 genes or expressed sequence tags (EST) randomly selected from a rat cDNA library. Results A total of 71 genes or ESTs were differently expressed in the cardiac muscles in 3 h after electrical injury with 35 up-regulated genes or ESTs and 36 down-regulated genes or ESTs. Some of these genes were involved in coagulation, inflammation, signal transduction and cell homeostasis. Conclusion The genomic response to heart electrical injury is complex and a great number of genes involved in this pathophysiological process. The analysis of differently expressed genes is helpful to learn the molecular mechanisms of heart electrical injury.