The antitumor activity of Shuang-Xi-Zuo-Wan-1 in C57/BL mice / 北京大学学报(医学版)

ABSTRACT

Objective: To detect the antitumor activity of Shuang-Xi-Zuo-Wan-1(Eb), a novel organoselenium compound, in C57/BL mice transplanted with Lewis lung cancer(LLC). Methods: The LLC transplanted C57/BL mice model was established,and the mice were randomly divided into four groups, including high dose Eb group (25.0 mg/kg),low dose Eb group (12.5 mg/kg), positive control group (DDP,2.0 mg/kg) and negative control group(solvent).Each group had twelve mice. Intraperitoneal injections (ip.) of four pharmaceuticals were performed once a day through the abdominal wall separately, from the second to the eighth days after cancer was transplanted. On the eleventh day,six mice of each group were killed and the influences of Eb on growth speed ,size, weight, invasion, inhibitory rate, proliferation index and apoptosis rate of LLC were observed and calculated.The remaining mice were fed till all of them died naturely and the average survival time of each group was calculated. Results:Eb could inhibit the growth and infiltration of LLC(the cancer inhibitory rate of high does Eb was 80.31%) obviously and prolong the average survival time of these with mice cancer. After being given Eb,the nuclear of the cancer cell concentrated and the fission phase cells reduced.In addition,the number of apoptosis cancer cells increased.Conclusion:The novel organoselenium compound Eb has antitumo activity in vivo .It can inhibit the growth and infiltration of LLC in mice, and induce the apoptosis of cancer cells.