Impaired islet ?-cell function and insulin resistance in adult male rats born with intrauterine growth retardation / 北京大学学报(医学版)

ABSTRACT

Objective: To investigate the islet ?-cell function and insulin sensitivity in adult male rats born intrauterine growth retardation caused by uterine placenta insufficiency. Methods: Bilateral uterine artery ligation (UAL) was performed on day 17 of gestation in the pregnant Wistar rats; sham-operated pregnant rats served as controls. The birth weights of the offspring in the UAL group were below the mean values of the birth weights of the control group more than 2SD, defined as IUGR. The tests were done in the adult male offspring (n=9 for each group). The glucose tolerance test was processed and Modified Beta-Cell function Index (MBCI) was calculated to evaluating the islet ?-cell endocrine function. Hyperinsulinemic- euglycemic clamp, the gold standard method to test the insulin resistance in the periphereal tissue was performed and insulin induced glucose infusion rate (GIR) was used to evaluate the insulin sensitivity. Results: (1) Though the mean birth weight of the newborn in the UAL group(4.49?0.56)g is much more than two standard deviations below the mean weight of that of the control group(6.16?0.30)g,P