Effects of irbesartan on expression of connective tissue growth factor in myocardium of spontaneously hypertensive rats / 中国临床药理学与治疗学

ABSTRACT

AIM: To investigate the expression of connective tissue growth factor (CTGF) in the myocardium of spontaneously hypertensive rats and the relationship of the hypertensive ventricular remodeling, myocardial fibrosis and whether irbesartan alter this expression. METHEDS 20 twenty-week-old male spontaneously hypertensive rats were randomly assigned to SHR group and irbesartan group (n=10 in each), and 10 twenty-week-old male Wistar Kyoto rats were considered as WKY group. Rats in irbesartan group were given a daily dose of 50 mg?kg~-1 irbesartan for 12 weeks by gavage and the other two groups were fed with a normal diet. TGF-?1 and CTGF were evaluated by qualitative and semiquantitative immunohistochemical staining and semiquantitative reverse transcription polymerase chain reaction. MASSON staining were used in the study of total collagen in left ventricular interstitial tissue. RESULTS: Compared with the WKY group, the left ventricular wight index(LVI), collagen volume fraction (CVF), perivascular collagen area (PVCA) and the expression of TGF-?1, CTGF and its mRNA in left ventricular increased in SHR group (P