HPLC method for the determination of huperzine A in Huperzia serrata / 中国药理学通报

ABSTRACT

Aim To establish a HPLC method for determination of the content of Huperzine A in Huperzia serrata and compare it in different parts of the plants. Method DiamonsilTm C_ 18 column(200 mm?4.6 mm,5 ?m)was used; the mobile phase was methanol ∶0.08 mol?L-1ammonium acetate buffer(40 ∶60,pH=6.0),the detection wavelength was 308 nm, with flow rate at 1.0 ml?min-1;the column temperature was room temperature.Results There is a good linear relationship within the range of 5～100 mg?L-1(r=0.9997).The average recovery was 99.10%,with RSD 2.44%(n=5).Regarding the content of Huperzine A in different parts of Huperzia serrata, it was found that the quantity of Huperzine A in leaf was higher than that in stem and it was the lowest in root. Conclusion The method is simple, accurate and reliable etition. Huperzine A can be accumulated in the aboveground part of the plant.