Hierarchical clustering the genes related to mouse optic nerve development and injury / 第三军医大学学报

ABSTRACT

Objective To find out the correlation among the genes which were screened by high density genechips related to mice optic nerve development and injury in our previous work,then to estimate the functions of some unknown genes according to some genes known related to CNS neurite extention and guidance.Methods Hierarchical clustering method was applied for the genes mentioned above and the subgroups respectively containing Ptn,Efnb3 were further analyzed.Results A total of 1 033 suitable candidate genes were clustered into 6 groups.The gene expression pattern of group B was identical to the development pattern of lateral geniculate body(LGN),which supposed that the genes of group B might be the key genes to the optic never development.Five functionally unknown genes Mm.28443,Mm.9671,Mm.25504,Mm.160640,Mm.182895 were clustered into a subgroup together with Ptn.The Pearson's coefficient between each of them and Ptn varied from 0.979 to 0.996.These genes were supposed to be candidate genes related to neurite growth and guidance.Lamr1 and its ligand were assumed to be the neurite guiding molecules for optic never,both beacuse of its high Coefficient to Efnb3 gene and related documents.Conclusion New neurite guiding molecules might be potential target genes for CNS regeneration by genetic engineering.