Construction of PGEFP-C1/N and its localization in transfected cell / 解放军医学杂志
Medical Journal of Chinese People's Liberation Army
;
(12)2001.
Article
in Chinese
| WPRIM
| ID: wpr-557334
ABSTRACT
Objective To identify the subcellular location of S AR S-CoV N protein in mammalian cells. Methods The gene fraction of SARS-CoV was cloned into the PGEFP-C1 plasmid to construct expression vecto r PGEFP-C1/N. The subcellular location of N in A549 and VeroE6 cells was observ ed under fluorescence microscope with the aid of transient transfection techniqu e. The expression of the fusion protein (GFP-N) was detected by Western blot. Results The PGEFP-C1/N was constructed. N protein was localiz ed in the cytoplasm of transfected cells and detected by Western blot. C onclusion N protein was localized in the cytoplasm of mammalian cells.
Full text:
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Medical Journal of Chinese People's Liberation Army
Year:
2001
Type:
Article
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