Increased expression of transforming growth factor-beta 1 in fetal liver stromal cells induced by hypoxia / 第二军医大学学报

ABSTRACT

Objective:To evaluate the expression of transforming growth factor-?1 (TGF-?1) in fetal liver stromal cells during hypoxia,in an attempt to provide evidences for cell therapy of brain ischemia. Methods: Cells from 14.5-day-old mouse fetuses were cultured and passaged. Subconfluent cells of the fifth passage were cultured in hypoxia condition (95% N2 + 5% CO2 ). TGF-?1 mRNA and TGF-?1 were detected by semi-quantitative RT-PCR and Western blot,respectively. Results: TGF-?1 mRNA and TGF-?1 levels,which are very low in normally cultured cells, increased significantly in hypoxia-cultured cells. Expression of TGF-?1 mRNA increased from (5.47?2.76)% to (10.38?3.65)% at 4 h of hypoxia(P