Therapeutic effect of vitamin E succinate combined with tamoxifen on graft model of human breast cancer in nude mice / 第二军医大学学报

ABSTRACT

Objective:To investigate the antitumor effect of vitamin E succinate combined with tamoxifen on the graft model of human breast cancer in nude mice and to analyze the possible mechanism.Methods: MCF-7 human breast cancer cells were inoculated subcutaneously in 40 nude mice.Then the mice were equally divided into 4 groups: blank control group(non-treatment),TAM treatment group(5 mg/kg),VES treatment group(150 mg/kg) and the TAM+VES treatment group(TAM 5 mg/kg +VES 150 mg/kg).The size of the tumor was measured and both the cell cycle and cell surface Fas/FasL were determined by flow cytometry 4 weeks after treatment.Fas/FasL expression in tumor tissue was detected with immunohistochemistry and cell apoptosis was detected by TUNEL method.Results: The co-administration of VES and TAM had more potent inhibitory effect on the growth of solid tumor than VES or TAM administered alone(P