Nitric oxide synthase inhibitors suppression against expression of ERK in spinal cord of morphine dependent and withdrawal rats / 中国药理学通报

ABSTRACT

Aim This study aimed to explore the effects of intrathecal injection of nitric oxide synthase inhibitors(L-NAME,7-NI) on morphine withdrawal response and the the spinal p-ERK expression.Methods Rats were divided into 5 groups control group,dependence group,withdrawal group, L-NAME group(L-NAME,it) and 7-NI group(7-NI,it).Morphine withdrawal score,touch evoked agitation scores(TEA scores),immunohistochemical and western-blotting technique were used to evaluate morphine withdrawal response and the expression of Fos and p-ERK in the spinal cord,respectively.Results Intrathecal injection of non-specific NOS inhibitor L-NAME,nNOS inhibitor 7-NI significantly alleviated morphine withdrawal symptoms.Morphine withdrawal scores in the L-NAME(22.1?4.52) and 7-NI groups(16.2?3.99) were significantly lower than that of the withdrawal group(28.6?4.89)(P