Study on X-ray induced frequency of micronucleated erythrocytes in mice bone marrow by flow cytometer / 第三军医大学学报

ABSTRACT

Objective To detect the frequency of micronucleated polychromatic erythrocytes(fMNPCE) in mice bone marrow after irradiated with X-ray by flow cytometer(FCM) to study whether fMNPCE detecting method could become a rapid biodosimeter for early radiation damage. Methods BALB/c mice were randomly assigned to receive the X-ray irradiation at dose of 0.5,3.0,6.0 Gy(n=4 for each irradiation dose),and the bone marrow was collected in 6,12,24 h after irradiation.Another 4 mice were intraperitoneally injected with cyclophosphamide twice as positive control,of which the bone marrow was collected in 42 h after intoxication began and 4 treated with spurious radiation as normal control.Results fMNPCE in mice bone marrow increased remarkably in 24 h after exposure to 0.5 Gy X-ray(P