Effect of photodynamic therapy with laser-activated BPD-MA on the expressions of mitochondria-related proteins Bcl-2 and Bax of human bladder cancer BIU-87 cells / 第三军医大学学报

ABSTRACT

Objective To study on the mechanisms that photodynamic therapy with laser-activated BPD-MA induces human bladder cancer BIU-87 cell apoptosis.Methods Human bladder cancer BIU-87 cells at log phase were randomized into 4 groups.The cells of BDP-MA-PDT groups were added with BPD-MA and then activated with 632.8 nm He-Ne laser;PDT group without BDP-MA addition was only irradiated;BDP-MA group without irradiation;normal control group without BDP-MA addition or irradiation.Photosensitization of BPD-MA was activated by laser with red light(632.8 nm) delivered at 10 mw/cm~(2) to give a total dose of 2.4 J/cm~(2).The expression of Bcl-2 and Bax proteins in human bladder cancer BIU-87 cells were detected by immunohistochemical staining.Results The Bcl-2 protein expression in BPD-MA-PDT group was lower than that of other groups(P0.05),therefore the ratio of Bax to Bcl-2 obviously increased(P