Study of manifestation of HCC on SPIO-enhanced MRI with pathologic basis in rats / 第三军医大学学报

ABSTRACT

Objective To investigate the manifestation of HCC on superparamagnetic iron oxide(SPIO)-enhanced MRI in rats and clarify its pathologic basis in hope of the application of SPIO-enhanced MRI in estimation of histological grading in HCC.Methods HCC was induced chronically with 1 ml of 2% DEN solution once a day in 80 rats for 14 weeks,and another 10 rats served as normal controls.All rats received dynamic MR examinations on week 16,18,20,21,22,23,24,25.MR imaging was performed before and after SPIO injection using SE-T1WI,FMPSPGR-T1WI,GRE-T2WI,FSE-PDWI,FSE-T2WI, and the relative parameters were obtained.Rats were killed as soon as MRI finished.The tumors were observed grossly and the parts matched with the lesions on MRI were cut into pieces,stained with HE and PerIs,for pathologic examination.Results Kupffer cell number in HCCs was significantly smaller than that in normal liver tissues(P