Relationship between anti-tumor necrosis factor -? monoclonal antibody and chronic colitis in mice / 中国实用内科杂志

ABSTRACT

Objective To investigate the role of tumor necrosis factor -?(TNF-?)in the pathogenesis of experimental murine colitis.Methods From Apr.1999 to May 2002,in the First Affiliated Hospital of Zhengzhou University,an experimental colitis model was established in which chronic colitis was induced by transfer of syngeneic CD45RB,CD~+_4T cells into severe combined immunodeficient(SCID)mice.These SCID recipients were treated with anti-TNF-? mAb,and the efficacy was observed and the mechanism was studied.Results Anti-TNF-? effectively prevented intestinal mucosal inflammation.Anti-TNF-? mAb also significantly suppressed leukocyte infiltration(e.g,CD~+_4 T cells,mononuclear macrophages)in the inflamed colon,and down-regulated production of proinflammatory cytokines interferon(IFN-?)and IL-2 of CD~+_4T cells in lamina propria.Conclusion The data suggests that administration of anti-TNF-? reverses mucosal inflammation via down-regulation of proinflammatory cytokines and decreases leukocyte infiltration in the bowel,thus providing additional support for TNF-?-targeted therapy in human Crohn's disease.