Protective effect of acteoside on rotenone-induced apoptosis in SH-SY5Y cells / 中国药理学通报

ABSTRACT

Aim To investigate whether acteoside can protect the dopaminergic(neurons,)(SH-SY5Y)(cells,) from rotenone-induced apoptosis.Methods Cell viability was analyzed by MTT assay.The neurons were stained with the fluorescent dye,Hoechst 33342,to analyze the nuclear change.Flow cytometry was used to determine neuronal apoptotic peak quantitatively.The level of intracellular reactive oxygen species(ROS) was monitored using the fluorescent probe 2′,7′-dichlorofluorescin-diacetate(DCFH-DA).Results ①SH-SY5Y cells treated with 0.5 ?mol ?L~(-1) rotenone for 48 h had a significantly decrease on cell viability compared with control,and the percentage of apoptosis was increased to 47.39%.The cell bodies of most cells shrinked and the dendrites of most cells became shorten or broken.Meanwhile,the shrinkage,condensation and cleavage of nuclei in most cells could be observed.The level of intracellular oxygen species was enhanced.② The preincubation with acteoside(10,20 or 40 mg?L~(-1)),one of the phenylethanoids isolated from Cistanche salsa,for 6 h enhanced the cell viability,and improved the rotenone-induced cell morphological change.Moreover,the percentage of apoptosis was significantly decreased to 25.87%,23.97% and 10.45% in a dose-dependent manner.The enhancement of ROS induced by rotenone was inhibited by 20 mg?L~(-1) acteoside.Conclusion These results demonstrate that acteoside can protect SH-SYSY cells against rotenone-induced apoptosis.The neuroprotective effect might be related with the function that acteoside can reduce the level of ROS.