Expression changes of alpha adrenergic receptors in heart and mesenteric artery in autonomic dysreflexia rats after spinal cord transection / 第二军医大学学报

ABSTRACT

Objective:To investigate the changes of alpha adrenergic receptors(?-AR) mRNA expression in heart and mesenteric artery in autonomic dysreflexia(AD) rats after spinal cord transection,so as to explore the possible mechanism of AD.Methods: The spinal cord of rats was exposed and the fourth thoracic spinal cord was transected;4 weeks later,rats' rectum was stimulated by self-made catheter and those with a mean arterial blood pressure increased by more than 15 mmHg(1 mmHg=(0.133) kPa) were chosen as AD group(n=16).Heart and mesenteric arteries along with their branches were harvested.mRNA expression of ?_(1A)-,?_(1B)-,?_(1D)-,?_(2A)-,?_(2B)-and ?_(2C)-AR was quantified by real time PCR and the result was compared with that in sham-operated group(the fourth thoracic spinal cord was exposed but not transected).Results: Compared with sham-operated group,rats in AD group had a lower expression of ?_(1A)-AR mRNA(P