Decrease of natural killer cell activity in patients with cryptococcosis and its clinical significance / 第二军医大学学报

ABSTRACT

Objective:To investigate the interactions between natural killer(NK) cells and Cryptococcus neoformans(C.neoformans) in patients with cryptococcosis,so as to pave a way for treatment and prevention of cryptococcosis.Methods: The peripheral blood samples of 40 cryptococcosis patients and 40 healthy controls were collected.Expression of CD2,CD3,CD4,CD8,CD28,CD18,CD19,and CD56 in patients peripheral blood mononuclear cells(PBMC) were detected by FACS.Cytotoxic activity of NK cells was analyzed by MTT using K562 cells as target cells and the influence of IFN-? and IL-2 on NK cell activity was also studied.The level of IFN-? in the culture supernatant was assayed by ELISA and the cytotoxic activity of the supernatant was determined.The transcription levels of perforin,granzyme B and granulysin were examined by quantative real-time PCR.C.neoformans and NK cells were cocultured to investigate the inhibition of NK cells on C.neoformans.Results: Compared with that in healthy controls,CD56~+ cells decreased significantly(P