Study on high-performance liquid chromatographic fingerprints of different germplasms of Flos Carthami / 第二军医大学学报

ABSTRACT

Objective:To study the high-performance liquid chromatographic(HPLC) fingerprints of different germplasms of Flos Carthami. Methods: HPLC was applied in chromatographic separation of 36 different germplasms of Flos Carthami collected from 11 provinces of China.The chromatography condition was Agilent Zorbax C_(18) column(4.6 mm?250 mm,5 mm);mobile phase CAN,0.5% H_3PO_4(from 1090 to 8020);running time 60 min;flow speed 1.0 ml/min;detection wavelength 265 nm;and temperature 20℃.The data were processed by Chromatographic Fingerprint Station designed by Department of Pharmaceutical Analysis,School of Pharmacy,Second Military Medical University.Different germplasms of Flos Carthami were subjected to cluster analysis and their similarity was also evaluated.Results: The different germplasms of Flos Carthami in this study were grouped into 2 chemical types.Samples from northwest of China were of high similarity;samples from the middle China were of high similarity to those from South China.The similarity of some germplasms of Flos Carthami was significantly different.Conclusion: The chemical components of different germplasms of Flos Carthami are obviously different from each other;selection of germplasm is very important in quality control of Flos Carthami.