Effects of Huaxian Capsule on expression of MMP-2/TIMP-1 Protein and MMP-2 mRNA in lung tissue of pulmonory fibrosis model in rats / 国际中医中药杂志

ABSTRACT

】 Objective To observe the effects and explore the mechanism of Huaxian Capsule (HXC) on pulmonary fibrosis. Methods 60 rats were divided into the normal group, the HXC group, the prednisone group, and the model group, 15 rats in each group. The pulmonary fibrosis model of rats was established with bleomycin. The experimental rats were sacrificed in batch on the 7th, 14th and 28th day for determining TIMP-1 protein and MMP-2 mRNA expression by immunohistochemistry and in situ hybridization respectively. The outcomes were compared between groups. Results It was showed that TIMP-1 protein and MMP-2 mRNA expression in different stage of pulmonary fibrosis increased significantly and continuously after modeling, showing significant difference in comparison between the model group and the normal group. After HXC treatment, the two indexes expressed weakly as compared with those in the model group, and showed significant difference to those in the model group on 14th and 28th. Conclusion HXC influence on the expression of TIMP-1 and MMP-2 mRNA in rats with pulmonary fibrosis. 【