The Changes of Serum Tumor Necrosis Factor and Epidermal Growth Factor in Rats with Experimental Liver Fibrosis / 医学研究杂志

ABSTRACT

Objective To study the the changes of serum tumor necrosis factor-?(TNF-?)and epidermal growth factor (EGF) in rats with experimental liver fibrosis and its significance. Methods All the SD rats were randomly divided into the normal group and the model group. The rats in the model group were subcutaneously injected with 40%CCl4 according to 0.3ml/100g body weight twice one week for the first four weeks, the initial dose was double. In the following five weeks, the rats were injected 40% CCl4 of 0.2ml/100g body weight twice one week.The rats in the normal group were subcutaneously injected with equal dosage of saline,mentioned aboveo.At the end of the ninth week,the serum was obtained,the levels of serum TNF-?, EGF were detected with radioimmunity, with HE stains, the severity of inflammation and fibrosis in all liver tissuses were measured by semiquantitative scoring. Results The fibrosis scorings, the levels of serum TNF-?, EGF in the model group were higher than that in the normal group, the difference between the two group was significance(P