Detection of differentially expressed genes between high-rate and low-rate metastatic human lung giant cell carcinoma cell strains using cDNA microarray / 第三军医大学学报

ABSTRACT

Objective To analyze the differences in gene expression between human lung giant cell carcinoma cell strains of high metastatic 95D and low metastatic 95C and to screen lung cancer metastasis-associated genes using cDNA microarray.Methods The mRNAs were extracted from human lung giant cell carcinoma cell strains of high metastatic 95D and low metastatic 95C,and reversely transcribed to the cDNAs and labeled by Cy5-dUTP and Cy3-dUTP to prepare the hybridization probes.The mixed probes were hybridized to the cDNA microarray chip.The information was obtained by managing the cDNA microarray chip with Scan Array 3000 scanner and ImaGene3.0 software.A part of genes whose expressions changed in cDNA microarray analysis were further identified by RT-PCR.Results The cDNA microarray analysis showed that the expressions of 466 genes changed,among which 108 pairs of Double Gene had the same GenBank ID and some of the genes,including Fln29,RUVBL2,C14orf3 et al,were further confirmed by RT-PCR.The results of RT-PCR was coincided well with the cDNA microarray results.Conclusion Many different genes are involved in the metastasis of lung cancer.cDNA microarray technique might be a useful method in screening lung cancer metastasis-associated genes.