HPLC-ELSD in determination of AR-Ⅰ content in total furostanol saponins of Asparagus cochinchinensis / 第二军医大学学报
Academic Journal of Second Military Medical University
;
(12)1981.
Article
in Chinese
| WPRIM
| ID: wpr-558641
ABSTRACT
Objective:
To determine AR-Ⅰ content in total furostanol saponins of Asparagus cochinchinensis.Methods:
High performance liquid chromatography(HPLC) was used and the condition was Diamonsil C_(18) column(5 mm?250 mm,5 ?m);mobile phase(CH_(3)CN-H_(2)O(36∶64);) flow rate 0.8 ml/min;injector volume20 ?l;colum temperature room temperature.Evaporative light scattering detector(ELSD) condition was drift tube temperature 40℃;and nebulizer gas pressure(N_(2)) 2.0?10~(5) Pa.Results:
The linearity was good when AR-I was within the range of 0.094-0.940 0 g?L~(-1)(r=0.999 2).The test had higher sensitivity and good stability.The average recovery was 101.3%(n=6)and RSD was 2.36%.Conclusion:
HPLC-ELSD is practical and reliable for the determination of the furostanol saponins of Asparagus cochinchinensis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
1981
Type:
Article
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