Morphological changes and differential protein spectrum of human malignant glioma cell SHG-44 after treated with Nordy,an analog of Nordihydroguaiaretic acid / 第二军医大学学报

ABSTRACT

Objective:To observe the morphological changes and analyze the differential protein spectrum of human malignant glioma cells SHG-44 after treated with Nordy(Chinese patent numberZL02133700.4),an analog of Nordihydroguaiaretic acid.Methods: The differentiation of SHG-44 cells was induced by 100 ?mol/L or 200 ?mol/L Nordy;the morphological changes of cells were observed 24,48 and 72 h after Nordy treatment and the findings were compared with those of the control group(received no treatment).The total proteins were extracted from SHG-44 cells treated with 200 ?mol/L Nordy for 72 h and cells in control group,then were subjected to two-dimensional gel electrophoresis.PDquest 7.1 software was employed to compare the protein expression differences.The highly expressed differential proteins were identified by matrix-assisted laser desorption/ionization-time of flight-mass spectrometry(MALDI-TOF-MS).Results: The morphological changes of SHG-44 cells treated with 200 ?mol/L Nordy were more obvious than those treated with 100 ?mol/L Nordy,and the most obvious differentiation was found in the cells treated for 72 h.Compared with those of control group,23 differential protein spots were identified by the two-dimensional electrophoresis,including 21 down-regulated ones and 2 up-regulated ones.MALDI-TOF-MS showed that the highly expressed proteins were an unknown protein,proliferation-associated gene A,Up1,alternative splicing factor ASF-3,cofilin1(non-muscle),eukaryotic translation initiation factor 5A,beta galactoside binding lectin,and glutathione-S-transferase Pi.Conclusion: Nordy can induce differentiation of human malignant glioma cells SHG-44 in a time-effect and dose-effect dependent manner.The Nordy-induced differential proteins may function in multiple aspects such as cell proliferation,apoptosis and gene transcription.