Human kidney proximal tubular epithelial cell line expresses paxillin induced by TGF-?_1 / 第三军医大学学报

ABSTRACT

Objective To investigate the expression of Paxillin (Pax) in human kidney proximal tubular epithelial cell line (HKC) induced by transforming growth factor ?1(TGF?1). Methods HKC cells cultured in vitro were divided into three groups at random control group(C) cultured with free serum medium(FSM), TGF?1-treated groups (T1 and T2) cultured with FSM containing different concentrations of TGF?1 (T1 5 ng/ml, T2 10 ng/ml). After 48-hour treatment, the expression of Pax mRNA and protein was detected by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemical staining in HKC cells respectively. Results In Group C, the expression of Pax mRNA and protein in HKC cells was elementary. However, in T1 and T2 groups , the expression of Pax mRNA and protein in HKC cells was greatly increased, especially in T2 group which was more significantly increased than T1 group (P