Effects of different magnitudes of mechanical strain on proliferation and alkaline phosphatase activity in osteoblast in vitro / 解放军医学杂志
Article
in Zh
| WPRIM
| ID: wpr-559099
Responsible library:
WPRO
ABSTRACT
Objective To investigate the effects of different magnitudes of mechanical strain on proliferation and alkaline phosphatase (ALP) activity in mouse osteoblast-like cells (MC3T3-E1). Methods MC3T3-E1 cells were subjected to 0%, 6%, 12%, 18% elongation for 24h and 48h by using multi-passage cell stress loading system respectively. MTT colorimetric method was used to assess the proliferation of the cell, ALP activity was detected by ALP assay kit. Results The proliferation of MC3T3-E1 cells was increased significantly 24h and 48h after mechanical strain treatment concomitant with increasing stretching force (P
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Index:
WPRIM
Language:
Zh
Journal:
Medical Journal of Chinese People's Liberation Army
Year:
1981
Type:
Article