Cloning of mouse gene BC023882 and its effect on cell cycle / 第三军医大学学报

ABSTRACT

Objective To obtain eukaryotic expression vectors containing coding region of the gene BC203882 and detect its effect on cell growth.Methods The coding region of the gene BC023882 was amplified by RT-PCR,then cloned into eukaryotic expression vector pcDNA3.1(-)/Myc-His(+)/lacZ.The recombinant plasmid was transfected into embryonic fibroblast of mouse and COS-7 cells with liposome method.Its effects on cell proliferation were examined by FACS and growth curves.Results The eukaryotic expression vectors containing coding region of the gene BC203882 were constructed.The growth of the transfected cells was retarded,and the proliferation index of cells was significantly decreased.Conclusion The eukaryotic expression vectors of the gene BC023882 were constructed successfully and the inhibitory effect of the gene on cell growth was observed.