Effects of ruscogenin on adhesion of HL-60 cells to ECV304 cells / 中国药理学通报

ABSTRACT

Aim To investigate the anti-inflammatory activity of ruscogenin,a major sapogenin from Ophiopogon japonicus,effect of ruscogenin on cell adhesion is studied.Methods By MTT assay,effect of ruscogenin on normal and TNF-?-induced adhesion of human pro-myelocytic leukemia cell strain(HL-60)to venous endothelial cell strain(ECV304)was observed and effect of ruscogenin on the viability of ECV304 cells and HL-60 cells were investigated.Results Adhesion between HL-60 cells and ECV304 cells induced by TNF-? was significantly inhibited when ECV304 cells were pretreated with ruscogenin at the concentration of 0.1,1.0 ?mol?L~(-1).Meanwhile,ruscogenin remarkably inhibited TNF-a-induced adhesion when HL-60 cells were pretreated with ruscogenin at the concentration of 0.001,0.01,0.1 ?mol?L~(-1).On the other hand,their viability and adhesion of HL-60 cells to normal ECV304 cells were not affected at above concentrations.Conclusion Ruscogenin shows significant anti-inflammatory activity via inhibition of adherence of HL-60 cells to activated ECV304 cells.